GCMS of Pesticides Template Experiment

  1. Sequence File:
  2. Data File: Guest Folder/Guest Data
  3. Methods File: Loyola Splitless_PTVG:

 

 

 

 

 

 

 

 

 

Great Lakes Instrumentation Collaboratory: Sharing Chemical Instrumentation Globally for Research and Education

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Remote Control Workshops
Pesticides: Content Modules
  1. Use of Organo Chlorine Pesticides
  2. Toxicology of OrganoChlorine Pesticides
  3. Endocrine Disruptors
  4. Risk Assessment of OCP
  5. Water Treatment and Sampling
  6. Environmental Chemistry of OCP
  7. Chemistry of Sample Prep.
  8. Landscape Sampling
  9. US EPA Method for OCP
  10. Gas Chromatography
  11. Mass Spectra of OC standards
  12. Step by Step Creation of Methods File
  13. Trace MS methods development
  14. Parent Ion Spectra
  15. Instrument Design
  16. Chemometrics
  17. Spatial Interpolation of Data
  18. Background on Remote Control
Run an Experiment
  1. Become a GLIC Member
  2. Read Content Modules 10-15
  3. Pass an On-Line Quiz
  4. Schedule a Training Experiment
  5. Download Virtual Private Network
  6. Access GCMS computer
  7. Run a Training Experiment
  8. Analyze Mass Spectra of Training Experiment Unknown
  9. Schedule the GCMS for your Experiment
  10. Prepare Your Samples
  11. Ship Your Samples
  12. Access the GCMS computer
  13. Upload your Excel Sample Data
  14. Open the Template Sequence File
  15. Run Your Experiment
Modified: July 18, 2007

AutoSampler:

  1. Sample volume 1 microliter
  2. Air volume 1 microliter
  3. Injection Delay 4 sec
  4. Pull-out delay 0 sec
  5. Injection Speed 50 microliter/sec
  6. Pull up speed 10 microliter/sec
  7. Pull ups: 3
  8. Volume 5 microliters
  9. Delay between pullups: 1 s
  10. Pre-injection rinses: 3
  11. Pre-injection rinse volume: 5 microliters
  12. Pre-injection rinse solvent: bottle A
  13. Post-injection rinses: 3
  14. Post-injection rinse volume: 5 microliters
  15. Post injection rinse solvent: bottle A

GC Conditions

  1. Initial Temp 100 oC
  2. Initial Hold Time 2 minutes
  3. Ramp 12C/minute to 280 C
  4. Hold 3 minutes at 280 C
  5. Maximum temp 350 C
  6. Prep Run Timeout 10 min
  7. Equilibration Time 0.5 min

Mass Spec Conditions

  1. Scan over mass range 45 to 400
  2. Scan from 6 to 20 minutes
  3. Scan at center of chromatographic peak
  4. MS scan every 20 microseconds
  5. Multiplier 250 EV
  6. Ionization mode EI+
  7. Source Temperature 200.00C
  8. Interface Temperature 275C